I. TRANSCRIPTION: ( 1 PT. EACH)
10. statFOR ANSWERS CLICK HERE.
I. IDENTIFICATION: ( 1 PT. EACH)
1. The supernatant fluid when clotted blood has been centrifuged.
2. The supernatant fluid when anti-coagulated blood has been centrifuged.
3. The reference method for cholesterol.
4. A process wherein a needle is introduced into a vein to obtain blood.
5. The simplest form of carbohydrate.
6. How many minutes do you centrifuge the blood for serum preparation?
7. It is the precision and accuracy of a method.
8. The reference method for glucose.
9. The normal pH of serum.
10. The most common sample for clinical chemistry.ANSWERS ARE FOUND IN THIS POST.
I. IDENTIFICATION: ( PT EACH)
1. Colorimetry- The method of determining your unknown concentration using the absorptivity coefficient of the substance.
2. Monochromator - The part of the basic components of the spectrophotometer that isolates the wavelength of interest.
3. Cu=Au/As X Cs - The formula for the ratio of unknown to standard.
4. Lambert's Law - The specific law that involves the depth of the solution in relation to the concentration of the solution.
5. Cuvette/cuvet - The container that holds the sample to be read in the spectrophotometer.
6. Its color - The specific property of the substance that the spectrophotometer measures.
7. Standard curve - The graph that is used to determine the unknown concentration.
8. Ultraviolet (uv)=below 350 nm, Visible light = 350 to 800 nm, infrared light - above 800 nm - The types and specific wavelength ranges of the types of light._______________________________
II. Matching type: Match column B with column A.
Column A Column B
1. Cell well incubator _b__ a. displays readings
2. Aspirator ____i__ b. utilizes 37 degrees temperature
3. Printer ___d___ c. contains the tube to be read
4. Display window __d,h____ d. gives a print out data of the readings
5. Keypads ___f___ e. where the sample is introduced
6. Cell well ___c___ f. where you manipulate the settings
g. where you test the samples.
h. displays the temperature
i. not in the list
Discuss briefly but comprehensively. ( 5 pts. Each)
1. Beer Lambert’s Law
2. Basic components of the spectrophotometer.
3. Universal precaution.
4. Precautions in using the spectrophotometer.
INCREASE B1, INCREASE TB = A
INCREASE B2, INCREASE TB = B
INCREASE B1 & B2, INCREASE TB = C
NONE OF THE ABOVE = D
SHADE CORRESPONDING BOXES TO THE LETTER OF YOUR CHOICE.
1. Obstruction of the biliary tract
2. Hepatic encephalopathy
4. Liver cirrhosis
5. Gilbert's Sydrome
7. Viral hepatitis
8. Infectious hepatitis
9. Stones in biliary duct
10. Hemolytic anemia
2. What is the Molarity of a 0.5 N H2SO4 solution? (5 pts.)
3. What is the dilution if you add 0.75 ml of serum to 3.0 ml of diluent? ( 5 pts.)
4. How would you prepare 1:20 serum dilution? (5 pts.)
5. How would you prepare a 0.5 % NaCl solution? (5 pts.)
6. You are asked to prepare 200 ml of the following standard concentrations from a 20 mg/dL standard stock solution: Tabulate volume of solute and solvent and also dilution. ( 5 pts. each)
A. 2 mg/dL C. 8 mg/dL
B. 4 mg/dL D. 10 mg/dL
E. 15 mg/dL
ANSWER TO QUESTION NO. 2
What is the Molarity of a 0.5 N H2SO4 solution?
Since the Normality is known = 0.5 N
All you have to do is to make use of the shorter formula which is
M = Normality divided by the valence or N/v (factor)
M = 0.5 / 2
M = 0.25 M of H2SO4 (ANSWER)
ANSWER TO QUESTION NO. 3
What is the dilution if you add 0.75 ml of serum to 3.0 ml of diluent?
Consider the volume of serum as 1 part.
The dilution is the number of parts of the solute in proportion to the diluents plus 1.
So if 1 part = 0.75 mL
Then 3 ml is = 4 parts ( 3 ml divided by 0.75 ml)
So 1 part serum = 0.75 ml
+ 4 parts diluents = 3.0 ml
Will give you 5 parts ( 1 part serum + 4 parts diluent = 5 parts)
Dilution then is
QUESTION NO. 4
How would you prepare 1:20 serum dilution?
You can assume several volumes in this question. You can assume 1 ml as the volume of the solute as it is the easiest.
1. So 1 ml of serum + 19 ml of diluent = 1:20 serum dilution.
2. You can also assume a smaller volume for serum, especially if you were not able to extract more blood. Let’s say you have 0.5 ml of serum and you have to prepare 1:20 dilution. All you have to do is to assume that 0.5 ml is one part.
3. If 0.5 ml is one part, and you need 19 parts of the diluents to prepare a 1;20 dilution; all you have to do is to multiply 19 parts with 0.5 ml to get the volume of the 19 parts.
4. Your answer would then be 9.5 ml. ( 19 X 0.5 = 9.5)
5. So 0.5 ml of serum + 9.5 ml of diluent = 1:20 serum dilution (ANSWER)
QUESTION NO. 5
How would you prepare a 0.5 % NaCl solution?
The formula for % (W/V) is
% = W divided by the total volume multiplied by 100.
0.5% = W/V X 100
Assume that the total volume is 100 ml if no volume is given, so by transposing we come up with this formula:
W = 0.5% X 100 (total volume) / 100 %
W = 0.5 grams per 100 ml of diluent (ANSWER)
Convert volumes to ml and weights to grams to facilitate computation.
For QUESTION NUMBER 6, the ANSWERS WILL BE GIVEN NEXT POST.