I. IDENTIFICATION: ( PT EACH)
1. Colorimetry- The method of determining your unknown concentration using the absorptivity coefficient of the substance.
2. Monochromator - The part of the basic components of the spectrophotometer that isolates the wavelength of interest.
3. Cu=Au/As X Cs - The formula for the ratio of unknown to standard.
4. Lambert's Law - The specific law that involves the depth of the solution in relation to the concentration of the solution.
5. Cuvette/cuvet - The container that holds the sample to be read in the spectrophotometer.
6. Its color - The specific property of the substance that the spectrophotometer measures.
7. Standard curve - The graph that is used to determine the unknown concentration.
8. Ultraviolet (uv)=below 350 nm, Visible light = 350 to 800 nm, infrared light - above 800 nm - The types and specific wavelength ranges of the types of light.
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II. Matching type: Match column B with column A.
Column A Column B
1. Cell well incubator _b__ a. displays readings
2. Aspirator ____i__ b. utilizes 37 degrees temperature
3. Printer ___d___ c. contains the tube to be read
4. Display window __d,h____ d. gives a print out data of the readings
5. Keypads ___f___ e. where the sample is introduced
6. Cell well ___c___ f. where you manipulate the settings
g. where you test the samples.
h. displays the temperature
i. not in the list
III. ESSAY.
Discuss briefly but comprehensively. ( 5 pts. Each)
1. Beer Lambert’s Law
2. Basic components of the spectrophotometer.
3. Universal precaution.
4. Precautions in using the spectrophotometer.
