Clinical Chemistry Review Questions

I. IDENTIFICATION: ( PT EACH)

1. Colorimetry- The method of determining your unknown concentration using the absorptivity coefficient of the substance.

2. Monochromator - The part of the basic components of the spectrophotometer that isolates the wavelength of interest.

3. Cu=Au/As X Cs - The formula for the ratio of unknown to standard.

4. Lambert's Law - The specific law that involves the depth of the solution in relation to the concentration of the solution.

5. Cuvette/cuvet - The container that holds the sample to be read in the spectrophotometer.

6. Its color - The specific property of the substance that the spectrophotometer measures.

7. Standard curve - The graph that is used to determine the unknown concentration.

8. Ultraviolet (uv)=below 350 nm, Visible light = 350 to 800 nm, infrared light - above 800 nm - The types and specific wavelength ranges of the types of light.

_______________________________

II. Matching type: Match column B with column A.

Column A Column B

1. Cell well incubator _b__ a. displays readings

2. Aspirator ____i__ b. utilizes 37 degrees temperature

3. Printer ___d___ c. contains the tube to be read

4. Display window __d,h____ d. gives a print out data of the readings

5. Keypads ___f___ e. where the sample is introduced

6. Cell well ___c___ f. where you manipulate the settings

g. where you test the samples.

h. displays the temperature

i. not in the list

III. ESSAY.

Discuss briefly but comprehensively. ( 5 pts. Each)

1. Beer Lambert’s Law

2. Basic components of the spectrophotometer.

3. Universal precaution.

4. Precautions in using the spectrophotometer.