GUIDE QUESTIONS -REPORTING

FLUOROMETRY

1. What type of light is measured in fluorometry?
a. fluorescent light emitted by analyte
b. light blocked by analytes in solution
c. emitted light by analyte
d. none of the above

2. A substance that does not fluoresce could be conjugated with other substances that
fluoresce to measure its concentration. This is termed as:
a. quenching
b. coupling method
c. anistropy
d. none of the above

3. It utilizes diffraction gratings to isolate wavelength of interest:
a. filter fluorometers
b. spectrofluorometers
c. either
d. neither

4. The light source of a fluorometer emits light:
a. of a shorter wavelength
b. of a longer wavelength
c. either
d. neither

5. The loss of energy and fluoresence due to collision caused by higher temperature is called:
a. quenching
b. coupling
c. fluorescence
d. none of the above

6. Fluorometry is how many times sensitive than spectrophotometry?
a. 100
b. 1,000
c. 10,000
d. 10

7. The two types of monochromators in fluorometry are:
a. excitation and emission
b. excitation and absorption
c. absorption and emission
d. emission and flourescence

8. This type of fluorometry that utilizes a secondary perpendicular intensity in polarization:
a. laser scanning
b. anisotropy
c. time resloved fluorometry
d. resonant energy transfer

9. It is the aspect of fluorometry that has been adapted to increase information content in the assay readout.

Select from the choices for no. 8

10. Co-localization of less than 200 nanometers can be determined by light microscopy alone:
a. true
b. false


ANSWERS

1. A
2. B
3. B
4. B
5. A
6. B
7. B
8. A
9. B
10. C