The concentration of the solution is directly proportional to the absorbance and inversely proportional to the transmitted light.
2. What is the relationship between the intensity of color of a solution to its concentration?
The intensity of color of a solution is directly proportional to its concentration.
3. What is the formula in solving the Cu?
Cu = Au X Cs/ As
Where: Cu -Concentration of the unknown
Au -Absorbance of the unknown
Cs -Concentration of the standard
As -Absorbance of standard
4. What are the other methods in computing for your unknown concentration?
a. Ratio of standard to known, using the formula in No. 3
b. Absorptivity value
c. Standard curve
5. Give the types of wavelength and the ranges by which they occur.
Ultraviolet (UV) - Below 350 nm
Visible - 350 to 800 nm
Infrared (IR) - above 800 nm
6. List the specific colors for each wavelength.
Blue - 475 nm
Green - 510 nm
Yellow - 570 nm
7. List and describe briefly the basic components of the spectrophotometer.
Light source- provides a continuous source of light.
Entrance slit - prevents entry of extraneous light.
Monochromator - selects the wavelength of choice in reading the unknown.
Exit slit - purifies and intensifies the light entering the cuvette.
Cuvette - contains the unknown solution.
Detector - detects the light absorbed or transmitted and converts it to a reading.
Readout device or digital display window - reflects the readings or concentration of the unknown solution.
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