1. Discuss the principle of the method.
ALT in the serum catalyzes the transfer of an amino acid group to a keto acid group to form pyruvate and L-glutamate. This is measured spectrophotometrically at 505-535 nanometers.
2. Aside from sodium hydroxide, what reagent could also be used to alkalinize the solution?
Potassium hydroxide
3. Why should the distilled water used in dissolving your NaOH pellets be CO2 free?
Because carbon dioxide can affect the transfer of the amino group to the keto acid group due to its carbon content. The carbon atom may act as an acceptor molecule in the reaction. This would falsely decrease your values.
4. Why do we use a semi-log graphing paper in plotting your calibration curve?
Because the it is simpler to use since the graphing paper will find the logarithms of the values beforehand.
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