Quiz, Exam Review Questions, Enzymes

1.____________________________________________ Ubiquitous enzyme.

2.____________________________________________ List the three cardiac isoenzymes.

3.____________________________________________ Enzyme primarily tested in acute pancreatitis.

4.____________________________________________The maximum temperature for enzyme activity to occur normally.

5.____________________________________________ The optimum pH for enzyme determinations.

6. .____________________________________________List the four(4) post obstructive enzymes.

7. .____________________________________________

8. .____________________________________________

9. .____________________________________________

10. – 12 Give three principles of enzyme data Interpretation.

____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

12 - 14. Give the three methods of determining enzyme activity

____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

15. .____________________________________________ The alcoholic marker enzyme.

16-20. Explain the electrophoretic mobility of CK.

READ THE ANSWERS HERE.

Laboratory Instrumentation Quiz

TEST I – Multiple Choice.

Choices for nos 1-5:
a. Pre-analytical
b. Analytical
c. Post-analytical

1. Exposed reagent strips
2. Outdated set of protocols
3. Illegible request forum
4. QNS
5. COC not properly followed

6. The resolving power of a microscope refers to its ability to
a. Refract light
b. Clearly show detail
c. Produce an enlarged image
d. Increase an object’s apparent size
e. All of these

7. What is the standard length of the draw tube of a microscope?
a. 10 cm
b. 160 mm
c. 1.4 dm
d. 3.5 inches
e. None of the above

8. If the image remains visible after the exchange of objectives, the lenses are said to be
a. Parfocal
b. Partentive
c. Parcentered
d. A & C
e. None of the above

9. What substances are best visualized using polarized light?
a. Birefringent substances
b. Doubly refractile substances
c. Substances that can refract light in two directions
d. A & C
e. A, B & C

10. Which is not true about the transmission electron microscope?
a. It is used to study viruses
b. It shows a detailed structure of larger microbes
c. Ultrathin sections of the specimen must be prepared
d. It provides a better view of the surface of even small microorganisms
e. Makes use of electron beams to produce contrast

QUIZ FOR CPK AND LDH

ESSAY:

1. DISCUSS THE MOST COMMON METHOD FOR ISOENZYME DETERMINATION FOR CPK AND LDH.

1.1. Illustrate the electrophoretic mobility of each isoenzyme.
1.2. How is the concentration measured afterwards?

2. WHAT ARE THE ISOENZYMES OF ALP? DISCUSS THE CHARACTERISTIC AND METHODS OF MEASUREMENT OF EACH ENZYME.

3. DISCUSS THE PRINCIPLES OF ENZYME DATA INTERPRETATION.

4. OUTLINE ACP AND ALP IN YOUR LECTURE NOTEBOOKS.


ANSWERS WILL BE POSTED NEXT WEEK.

Blood Gas Analysis - Review Questions

Name: Clinical Chemistry 2 -Blood Gas Analysis

Modified True/False Indicate whether the statement is true or false. If false, change the identified word or phrase to make the statement true.
T F	1.	Blood Gas Analysis is considered a routine test in clinical laboratories . This is because it is related to several common diseases.
T F	2.	Partial pressure of oxygen (pO2) measurements determines the ability of the lungs to “oxygenate” the body.
T F	3.	When computing for the pH values, carbonic acid is considered as dissolved carbon dioxide.
T F	4.	The normal pH of arterial blood ranges from, 7.45-7.55.
T F	5.	The normal ratio of carbonic acid to bicarbonate is 1:20.
T F	6.	Respiratory alkalosis is usually demonstrated by increased blood bicarbonate levels.
T F	7.	Hyperventilation causes acidosis because of the rapid excretion of carbon dioxide.
T F	8.	When there is respirtory alkalosis, the kidneys will respond by increasing the exretion of bicarbonates.
T F	9.	The best specimen for Blood Gas Analysis is heparinized blood.
T F	10.	The lungs and the kidneys are the organs involved in maintaining normal blood pH.
Matching
		a. respiratory alkalosis e. all of the above b. respiratory acidosis f. none of the above c. metabolic alkalosis g. a & b only d. metabolic acidosis h. c & d only
A B C D E F G H	11.	diarrhea
A B C D E F G H	12.	bronchial asthma
A B C D E F G H	13.	polyuria
A B C D E F G H	14.	hypoventilation
A B C D E F G H	15.	strenuous exercise
A B C D E F G H	16.	carbon dioxide is involved
A B C D E F G H	17.	bicarbonate is involved
A B C D E F G H	18.	pH is 8.5 with a HCO3 value as 23 mmol/L
Problem
	19.	A 20 year old female patient was admitted to the emergency ward. She complained of nausea, vomitting and polyuria. Her laboratory results were: Hemoglobin - 13 mg/dL White blood cells - 6,245 /cumm FBS - 70 mg/dL BUA - 3 mg/dL BUN - 15 mg/dL Serum creatinine - 1.2 mg/dL TAG - 140 mg/dL Cholesterol - 200 mg/dL pCO2 - 38 mmHg HCO3- 15 mmol/L Answer the following questions: 1. What is the acid base status of the patient? 2. What are the abnormal results in her laboratory tests? 3. What is the pH of the blood? 4. What further tests can be performed?
Essay
	20.	Discuss the compensatory mechanism of the body when there is metabolic alkalosis.