ACP and ALP, LDH Quiz in Clinical Chemistry

ACP and ALP, LDH

Name:____________________________________________ Rating__________________

Date:_____________________________________________ Section:_________________

1. __________________________________________ The best sample for ACP determination. (specify)

2. __________________________________________The best anticoagulant for ACP determination.(Specify).

3. __________________________________________Indicate what are the major diseases involve in ALP elevation.

4. __________________________________________ Normal value for ACP.

5. __________________________________________Indicate the inhibiting substance for PAP test in isoenzyme immunoassays.

6. __________________________________________ Give the specific reliable method for PAP and its substrate.

7. Three MAJOR specific precautions for ALP test. ___________________________________________

8. ___________________________________________________________________________________

9. ___________________________________________________________________________________

10. __________________________________________ The end product in ACP test.

11. to 15. General reaction that occurs in ACP tests using PNPP substrate. Answer at the back

16. to 20. General reaction catalyzed by ALP. Answer at the back.

21 . The amount of LDH inside the red blood cell___________________.

.22 to 25. The General reaction for LDH Determinations.

25 to 30. Discuss the isoenzymes of ALP and their clinical significance.

Answers to Clinical Chemistry 1 Review Questions

I. IDENTIFICATION: ( 1 PT. EACH)

1. The supernatant fluid when clotted blood has been centrifuged. SERUM

2. The supernatant fluid when anti-coagulated blood has been centrifuged. PLASMA

3. The reference colorimetric method for cholesterol. SCHOENHEIMER AND SPERRY

4. A process wherein a needle is introduced into a vein to obtain blood. VENIPUNCTURE/PHLEBOTOMY

5. The simplest form of carbohydrate. MONOSACCHARIDE

6. How many minutes do you centrifuge the blood for serum preparation? 5-10 MINUTES

7. It is the precision and accuracy of a method. RELIABILITY

8. The reference method for glucose. HEXOKINASE

9. The normal pH of serum. 7.35 TO 7.45 WITH AN AVERAGE OF 7.4

10. The most common sample for clinical chemistry. UNHEMOLYZED, NON-ICTERIC, NON-LIPEMIC, CLEAR SERUM