Review Questions on Carbohydrates, Lipids and Proteins

CLINICAL CHEMISTRY Review Questions Lipids, CHO, CHON

1.      _________________________________________ The lipid that acts as a lung surfactant.

2.      _________________________________________ The most predominant monosaccharide.

3.      _________________________________________ The simplest form of lipids.

4.      _________________________________________ The storage form of glucose.

5.      _________________________________________ The storage form of lipids.

6.      _________________________________________ The major cause of diabetes mellitus.

7.      _________________________________________ The standard fasting hours for FBS.

8.      _________________________________________ Transcribe FBS.

9.      _________________________________________ The percentage that glucose is destroyed per hour at room temperature.

10.  _________________________________________ The reference method for glucose.

11.  _________________________________________ The ideal anticoagulant for glucose.

12.  _________________________________________ Name one function of proteins.

13.  _________________________________________ Ideal fasting hours for TAG.

14.  _________________________________________ Transcribe TAG.

15.  _________________________________________ Ideal fasting hours for protein.

16-20. Draw the different layers in plasma and serum preparation after centrifugation.

21.25. Discuss how insulin and glucagon maintain normal blood glucose levels.

Bonus: _________________________________________ Name the basic nucleus of cholesterol.

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Answers to Review Questions on Lab Instrumentation

NASWERS TO Matching Type (Match column A with column B)

(Column A) 


1. Reflectance spectrophotometry - I

2. Absorbance or transmittance - G

3. Emitted light - C

4. Electromagnetic radiation at ground state - N

5. Stationary and mobile phase - B

6. Freezing point depression - A

7. Reaction of antigen –antibody - J

8. Longer wavelength - D

9. C1V1 = C2V2 - H

10. Rotor - K


 (Column B)


A. OSMOMETRY

B. CHROMATOGRAPHY

C. FLAME PHOTOMETRY

D. FLUOROMETRY

E. GRAVIMETRY

F. TITRIMETRY

G. SPECTROPHOTOMETRY

H. VOLUMETRY

I. DRY SLIDE TECHNOLOGY

J. IMMUNOASSAY

K. CENTRIFUGAL ANALYSIS

L. DUPONT ACA

M. SEQUENTIAL ANALYSIS

N. NIL



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Sample Review Questions on Laboratory Instrumentation

Matching Type (Match column B with column A 

(Column A) 


1. Reflectance spectrophotometry

2. Absorbance or transmittance

3. Emitted light

4. Electromagnetic radiation at ground state

5. Stationary and mobile phase

6. Freezing point depression

7. Reaction of antigen –antibody

8. Longer wavelength

9. C1V1 = C2V2

10. rotor


 (Column B)


A. OSMOMETRY

B. CHROMATOGRAPHY

C. FLAME PHOTOMETRY

D. FLUOROMETRY

E. GRAVIMETRY

F. TITRIMETRY

G. SPECTROPHOTOMETRY

H. VOLUMETRY

I. DRY SLIDE TECHNOLOGY

J. IMMUNOASSAY

K. CENTRIFUGAL ANALYSIS

L. DUPONT ACA

M. SEQUENTIAL ANALYSIS

N. NIL



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Answers to Normality, Molarity Sample Review Problems

1. What is the Normality of a 1.2 M H2SO4 solution given the molecular weights?( H=1, S=32, O-16)

    a. 1.2 N                   b. 0.6N                        c. 10.4 N                  d. 2.4 N              e. NIL

2. How many grams of NaCl is used to prepare 500 ml of a 0.5 N solution of NaCl? (Na-23, Cl-35.5)

    a. 14.63  g               b. 56.5 g                      c. 40 g             d. 13.8 g          e. NIL

3. What is the Molarity of a 3 .5 N solution of NaCl?

a.       2.50 mol/l                   c. 1.5 mol/l                   e. NIL

b.      3.5 mol/l                      d. 4.5  mol/l

4. The molecular weight of  H3 PO4 is:

a. 98                            c. 98                            e. NIL

b. 48                            d. 98.1

5. How many grams of  NaCl are required to make 2,000 ml. of  0.2 M  solution?

a. 32.3                                     c. 46.32                       e. NIL

b. 23.4                         d. 40

6. What is the Molar concentration of a 80 grm. of NaOH diluted to 1.5 liter of distilled water?

a. 1.33 M                                                        c. 1.5 M                       e. NIL

b. 1 M                                                              d. 3 M

  7. What is the amount of CaCl2.H2O in grams is needed  to prepare 0.2 N solution of

Ca Cl2?

a. 6.8                                                              c. 32                            e. NIL

b. 8                                                                  d. 55       

  8. One milligram is equal to:

a. 0.00001 grm.                                               c. 0.01 grm.                 e. NIL

b. 0.0001 grm.                                                 d. 0.001 grm.

 9. A 20 mgs. % solution contains:

a.      20 mgs of solute/100ml of solution

b.      20 mgs of solute/100 ml of diluent 

c.       20 mgs of solute/1000 ml of diluent

d.      20 mgs of solute/1000ml of solution

e.       NIL

10. A 6 % solution of 1mg/100ml is diluted 1:20. What is the final concentration?

a. 12 %                                                            c. 0.12 %                     e. NIL

b. 0.02 %                                                         d. 0.03 %

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Review Questions on Normality and Molarity

1. What is the Normality of a 1.2 M H2SO4 solution given the molecular weights?( H=1, S=32, O-16)

    a. 1.2 N                   b. 0.6N                        c. 10.4 N                  d. 2.4 N              e. NIL

2. How many grams of NaCl is used to prepare 500 ml of a 0.5 N solution of NaCl? (Na-23, Cl-35.5)

    a. 14.63  g               b. 56.5 g                      c. 40 g             d. 13.8 g          e. NIL

3. What is the Molarity of a 3 .5 N solution of NaCl?

a.       2.50 mol/l                   c. 1.5 mol/l                   e. NIL

b.      3.5 mol/l                      d. 4.5  mol/l

4. The molecular weight of  H3 PO4 is:

a. 98                            c. 98                            e. NIL

b. 48                            d. 98.1

5. How many grams of  NaCl are required to make 2,000 ml. of  0.2 M  solution?

a. 32.3                                     c. 46.32                       e. NIL

b. 23.4                         d. 40

6. What is the Molar concentration of a 80 grm. of NaOH diluted to 1.5 liter of distilled water?

a. 1.33 M                                                         c. 1.5 M                       e. NIL

b. 1 M                                                              d. 3 M

  7. What is the amount of CaCl2.H2O in grams is needed  to prepare 0.2 N solution of

Ca Cl2?

a. 6.8                                                              c. 32                            e. NIL

b. 8                                                                  d. 55

  8. One milligram is equal to:

a. 0.00001 grm.                                               c. 0.01 grm.                 e. NIL

b. 0.0001 grm.                                                 d. 0.001 grm.

 9. A 20 mgs. % solution contains:

a.       20 mgs of solute/100ml of solution

b.      20 mgs of solute/100 ml of diluent 

c.       20 mgs of solute/1000 ml of diluent

d.      20 mgs of solute/1000ml of solution

e.       NIL

10. A 6 % solution of 1mg/100ml is diluted 1:20. What is the final concentration?

a. 12 %                                                            c. 0.12 %                     e. NIL

b. 0.02 %                                                         d. 0.03 %

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CLICK HERE FOR LECTURE NOTES ON NORMALITY AND MOLARITY.

Answers to Electrolyte and Instrumentation Review Questions

1. In electrophoresis, the basis of separation are the following, EXCEPT:

a. Charges of ions
b. Electrophoretic media
c. Net charge of particles
d. Concentration of ions
e. NIL

2. The movement of ions in electrophoresis is from:
a. Negative to positive
b. Positive to negative
c. Negative to negative
d. Positive to positive
e. NIL

3. The principle of chromatography is:
a. The movement of charged particles in an electric field
b. The involvement the solute and solvent
c. The measurement of emitted light
d. The excitation of ions in solution
e. NIL

4. Chromatography is affected by the following factors, EXCEPT:
a. Density of particles in solution
b. Size of particle
c. Affinity of particles to chromatographic media
d. pH
e. NIL

5. The following precautions are observed in Gravimetry, EXCEPT:
a. The balance should be adjusted to zero
b. The substance to be measured should be hydrated
c. The substance should be uncontaminated
d. The vessels used should be clean and dry
e. NIL

6. The specific substance that causes lipemia is:
a. Lipoprotein lipase
b. Chylomicrons
c. Triglycerides
d. Cholesterol
e. NIL

7. The most predominant carbohydrate is:
a. Fatty acid
b. Glucose
c. Amino acid
d. Triglyceride
e. NIL

8. Chromatography can be utilized in the separation of the following, EXCEPT:
a. Proteins
b. Sugars
c. Drugs
d. Glucose
e. NIL

9. The following are effects of long standing of serum samples not separated from the cells at room temperature in glucose determination:
1. There will be falsely decreased results.
2. The rate of loss of glucose due to long standing at RT is 7 mg/dL/hr. if the serum is not separated from the cells.
3. The rate of loss of glucose may be higher due to possible bacterial contamination.
4. There will be falsely elevated values.
5. There will be no effect on glucose values.

a. 1
b. 1 and 2
c. 1, 2 and 3
d. 1, 3 and 5
e. NIL

10. Convert 6.5 mmol/L of glucose to traditional units.
a. 122.78
b. 123.56
c. 119.07
d. 120.72
e. NIL

11. Which of the following is the reference method for glucose?
a. Somogyi-Nelson
b. Hexokinase
c. Orthotoluidine
d. Nelson Somogyi
e. NIL

12. Which of the following is a characteristic of Type 1 DM
a. Family history of DM
b. Usually occurs at at age 40 and above
c. Ketosis not prominent
d. Thin persons
e. NIL  

Review Exam Questions in Clinical Chemistry 1

1. In electrophoresis, the basis of separation are the following, EXCEPT:

a. Charges of ions
b. Electrophoretic media
c. Net charge of particles
d. Concentration of ions
e. NIL

2. The movement of ions in electrophoresis is from:
a. Negative to positive
b. Positive to negative
c. Negative to negative
d. Positive to positive
e. NIL

3. The principle of chromatography is:
a. The movement of charged particles in an electric field
b. The involvement the solute and solvent
c. The measurement of emitted light
d. The excitation of ions in solution
e. NIL

4. Chromatography is affected by the following factors, EXCEPT:
a. Density of particles in solution
b. Size of particle
c. Affinity of particles to chromatographic media
d. pH
e. NIL

5. The following precautions are observed in Gravimetry, EXCEPT:
a. The balance should be adjusted to zero
b. The substance to be measured should be hydrated
c. The substance should be uncontaminated
d. The vessels used should be clean and dry
e. NIL

6. The specific substance that causes lipemia is:
a. Lipoprotein lipase
b. Chylomicrons
c. Triglycerides
d. Cholesterol
e. NIL

7. The most predominant carbohydrate is:
a. Fatty acid
b. Glucose
c. Amino acid
d. Triglyceride
e. NIL

8. Chromatography can be utilized in the separation of the following, EXCEPT:
a. Proteins
b. Sugars
c. Drugs
d. Glucose
e. NIL

9. The following are effects of long standing of serum samples not separated from the cells at room temperature in glucose determination:
1. There will be falsely decreased results.
2. The rate of loss of glucose due to long standing at RT is 7 mg/dL/hr. if the serum is not separated from the cells.
3. The rate of loss of glucose may be higher due to possible bacterial contamination.
4. There will be falsely elevated values.
5. There will be no effect on glucose values.

a. 1
b. 1 and 2
c. 1, 2 and 3
d. 1, 3 and 5
e. NIL

10. Convert 6.5 mmol/L of glucose to traditional units.
a. 122.78
b. 123.56
c. 119.07
d. 120.72
e. NIL

11. Which of the following is the reference method for glucose?
a. Somogyi-Nelson
b. Hexokinase
c. Orthotoluidine
d. Nelson Somogyi
e. NIL

12. Which of the following is a characteristic of Type 1 DM
a. Family history of DM
b. Usually occurs at at age 40 and above
c. Ketosis not prominent
d. Thin persons
e. NIL  

HERE ARE the CORRECT ANSWERS.