2. CK2MB, AST, LD1,___ List the three cardiac isoenzymes.
3. AMYLASE__ Enzyme primarily tested in acute pancreatitis.
4. 40 DEGREES C__The maximum temperature for enzyme activity to occur normally.
5. 7.4___ The optimum pH for enzyme determinations.
6. .ALKALINE PHOSPHATASE (ALP)_List the four(4) post obstructive enzymes.
7. . 5' NUCLEOTIDASE_(5' NT)_
8. . LEUCINE AMINO PEPTIDASE_(LAP)
9. .GAMMA GLUTAMYL TRANSFERASE_(GGT)
10. – 12 Give three principles of enzyme data Interpretation.
A. ENZYMES ARE FOUND WITHIN CELLS, SO ANY ELEVATION IN THEIR BLOOD CONCENTRATION WOULD INDICATE CELLULAR DAMAGE OR NECROSIS.
B. NORMAL RESULTS ARE SIGNIFICANT ( DO A SERIES OF TESTS.)
C. INTEGRATE ALL DATA BEFORE DIAGNOSIS, (PHYSICAL SYMPTOMS, OTHER DIAGNOSTIC EXAMS, HISTORY, ETC)
12 - 14. Give the three general methods of determining enzyme activity
A. MEASUREMENT OF ENZYME ACTIVITY
B. MEASUREMENT OF PRODUCT FORMED
C. MEASUREMENT OF DISAPPEARANCE OF SUBSTRATE
15. .GAMMA GLUTAMYL TRANSFERASE (GGT)_ The alcoholic marker enzyme.
16-20. Explain the electrophoretic mobility of CK.
IT IS THE MOVEMENT OF THE CK ISOENZYMES BASED ON THEIR CHARGES, SIZE, AFFINITY TO MEDIA AND THE NET CHARGE OF THE ELECTROPHORETIC FIELD. CKBB OR CK1 IS THE FASTEST FOLLOWED BY CK2 OR CKMB AND THEN CK 3 OR CKMM.
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