Answers to Review Questions on Lipids, Carbohydrates and Proteins

1.      ____________LIPOPROTEINS_______________ The lipid that acts as a carrier protein.

2.      ____________TRIGLYCERIDE______________ The storage form of lipids or fats in the body.

3.      _____________AMINO ACIDS______________ The simplest form of proteins.

4.      _____________GLYCOGEN ________________ The storage form of glucose.

5.      _____________AMINO ACIDS______________ The storage form of proteins.

6.      _____INSUFFICIENCY OF ADH_____________ The major cause of diabetes insipidus.

7.      ____NONE - BUT WHEN  IT’S DONE WITH TAG – 12 TO 14 HOURS__________________________________ The standard fasting hours for cholesterol.

8.      ____RANDOM BLOOD SUGAR_____________ Transcribe RBS.

9.      ____ 1 to 2 %_____________________________ The percentage that glucose is destroyed per hour at refrigerated temperatures.

10.  _____LIEBERMANN BURCHARD/T_________ The reference method -cholesterol.

11.  _____BUILDING BLOCKS OF THE BODY____ Name one function of proteins.

12.   _____SODIUM FLUORIDE_________________ Ideal anticoagulant for glucose.

13.  _______12 to 14 HOURS ___________________  Ideal fasting hours for TAG.

14.  ______TRIACYLGLYCEROL, TRIGLYCEIDE_ Transcribe TAG.

15.  ______NONE, BUT AS A MATTER OF PROTOCOL 8 to 12 HOURS_ Ideal fasting hours for protein test.

Review Questions on Lipids, Carbohydrates and Proteins

1.      _________________________________________ The lipid that acts as a carrier protein.

2.      _________________________________________ The storage form of lipids or fats in the body.

3.      _________________________________________ The simplest form of proteins.

4.      _________________________________________ The storage form of glucose.

5.      _________________________________________ The storage form of proteins.

6.      _________________________________________ The major cause of diabetes insipidus.

7.      _________________________________________ The standard fasting hours for cholesterol.

8.      _________________________________________ Transcribe RBS.

9.      _________________________________________ The percentage that glucose is destroyed per hour at refrigerated temperatures.

10.  _________________________________________ The reference method -cholesterol.

11.  _________________________________________ Name one function of proteins.

12.   _________________________________________ Ideal anticoagulant for glucose.

13.  _________________________________________  Ideal fasting hours for TAG.

14.  _________________________________________ Transcribe TAG.

15.  _________________________________________ Ideal fasting hours for protein test.

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Answers to Review Questions on Renal Function Test (RFT)

1.      ___BUN_____________________ The 2 major substances measured in blood for Renal Function test.

2.      ___CREATININE_____________

3.      ____2.14_____________________ The conversion factor of Blood Urea Nitrogen (BUN) to urea.

4.      ____88.4______________________ The conversion factor of creatinine from traditional units to SI units.

5.      ____0.357_____________________The conversion factor of BUN from traditional units to SI units.

6.      ____0.059_____________________ The conversion factor of BUA from traditional units to SI units.

7.      ____BUA_____________________ The end product of purine catabolism.

8.      ____CREATININE_____________ The end product of muscle metabolism.

9.      ____BUN_____________________ The end product of protein metabolism.

10.  ____BLOOD URIC ACID_______ Transcribe BUA.

Review Questions in Renal Function Test (RFT)

1.      ____________________________ The 2 major substances measured in blood for Renal Function test.

2.      ____________________________

3.      ____________________________ The conversion factor of Blood Urea Nitrogen (BUN) to urea.

4.      ____________________________ The conversion factor of creatinine from traditional units to SI units.

5.      ____________________________The conversion factor of BUN from traditional units to SI units.

6.      ____________________________ The conversion factor of BUA from traditional units to SI units.

7.      ____________________________ The end product of purine catabolism.

8.      ____________________________ The end product of muscle metabolism.

9.      ____________________________ The end product of protein metabolism.

10.  ____________________________ Transcribe BUA.


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Answers to Laboratory Instrumentation Review Questions

      1.      It is the law applied to spectrophotometry:

a.  Beer-Lambert’s Law                                  c.  Kirchoff’s Law

b.  Newton’s Law                                           d.  Absorptivity coeffecient

                                                                                                e. NIL

2.      The following are basic components of the  spectrophotometer, except;
                        a.  burner assembly                                         c.  cuvette                    e. NIL

                        b.  monochomator                                           d.  exit slit

3.The more light absorbed, the higher  the concentration of analyte in this technique of measuring the amount of light absorbed  by a solution:

a.    atomic absorption                                      d. fluorometry            

b.    nephelometry                                            e. NIL

c.    spectrophotometry                                   

4.      The basis of EFP  is the measurement of:

a.       light given off by excited atoms

b.      light absorbed at wavelength of resonance line by dissociated atoms

c.       energy emitted by ultraviolet treated atoms

d.      energy emitted by infrared treated atoms

e.       NIL

5. 2- log T is a definition of (T= transmission):

    a. absorbance                                              d. electron density                  e. NIL

     b. ionic strength                                         e. electrophoretic density

6.  In Osmometry, concentration of substance can be measured by:

   a. colorimetry                                              d. freezing point depression

    b. appropriate filters                                    e. NIL

    c. ionized atoms

7.  Quenching is a problem in analysis of:

   a. ultraviolet  spectrophotometry                 d. flame photometry

   b. fluorescence                                             e. NIL

   c. atomic absorption

8.  For AAS measurements the light source is usually the:

a. hollow cathode lamp                                    d. Tungsten light bulb

b. Deuterium lamp                                            e. NIL

c. halogen lamp

9.  Electrophoresis is used to measure :

a.       ultraviolet substances                                      e. NIL

b.      infrared absorbing materials

c.       charged particles

d.      colored solutions

10.  The reagent blank corrects for absorbance caused by:

a.       the color of reagents                                       e. NIL

b.      sample turbidity

c.       bilirubin and hemolysis

d.      all of the above

11.  The internal standards in EFP, are:

1. Didymium and lithium
2. Cesium and lithium
3. Potassium and didymium
4. Cesium and didymium
5. NIL

12.  The parts of the burner assembly are the following:

1. Aspirator, atomizer, flame
2. Aspirator, ionizer, flame
3. Atomizer, emitter, flame
4. Atomizer, ionizer, flame
5. NIL

13.  The most accurate vessel in volumetric measurements is the:

a.       Erlenmeyer flask

b.      Graduated cylinder

c.       Serological pipette

d.      Volumetric flask

e.       NIL

14.  Volumetric measurements are generally used in:

1. Liquid preparations only
2. Solid and liquid preparations
3. Solid preparations only
4. AIL
5. NIL

15.  The unit of volume in volumetric measurements is:

1. Deciliter
2. Micriolter
3. Milliliter
4. Liter
5. NIL

16.  In electrophoresis, the basis of separation are the following, EXCEPT:

1. Charges of ions
2. Electrophoretic media
3. Net charge of particles
4. Concentration of ions
5. NIL

17.  The movement of ions in electrophoresis is from:

1. Negative to positive
2. Positive to negative
3. Negative to negative
4. Positive to positive
5. NIL

18.  The principle of chromatography is:

1. The movement of charged particles in an electric field
2. The involvement the solute and solvent
3. The measurement of emitted light
4. The excitation of ions in solution
5. NIL

19.  Chromatography is affected by the following factors, EXCEPT:

1. Density of particles in solution
2. Size of particle
3. Affinity of particles to chromatographic media
4. pH
5. NIL

20.  The following precautions are observed in Gravimetry, EXCEPT:

1. The balance should be adjusted to zero
2. The substance to be measured should be hydrated
3. The substance should be uncontaminated
4. The vessels used should be clean and dry
5. NIL

21.  The specific substance that causes lipemia is

1. Lipoprotein lipase
2. Chylomicron
3. Triglycerides
4. Cholesterol
5. NIL

22.  The most predominant carbohydrate is:

1. Fatty acid
2. Glucose
3. Amino acid
4. Triglyceride
5. NIL

23.  Chromatography can be utilized in the separation of the following, EXCEPT:

1. Proteins
2. Sugars
3. Drugs
4. Glucose
5. NIL

24.  The following are effects of long standing of serum samples not separated from the cells at room temperature in glucose determination:

1.      There will be falsely decreased results.

2.      The rate of loss of glucose due to long standing at RT is 7 mg/dL/hr. if the serum is not separated from the cells.

3.      The rate of loss of glucose may be higher due to possible bacterial contamination.

4.      There will be falsely elevated values.

5.      There will be no effect on glucose values.

a.       1          b. 1 and 2        c. 1, 2 and 3    d. 1, 3 and 5    e. NIL

Review Sample Questions in Lab Instrumentation

      1.      It is the law applied to spectrophotometry:

a.  Beer-Lambert’s Law                                  c.  Kirchoff’s Law

b.  Newton’s Law                                           d.  Absorptivity coeffecient

                                                                                                e. NIL

2.      The following are basic components of the  spectrophotometer, except;
                        a.  burner assembly                                         c.  cuvette                    e. NIL

                        b.  monochomator                                           d.  exit slit

3.The more light absorbed, the higher  the concentration of analyte in this technique of measuring the amount of light absorbed  by a solution:

a.    atomic absorption                                      d. fluorometry            

b.    nephelometry                                            e. NIL

c.    spectrophotometry                                   

4.      The basis of EFP  is the measurement of:

a.       light given off by excited atoms

b.      light absorbed at wavelength of resonance line by dissociated atoms

c.       energy emitted by ultraviolet treated atoms

d.      energy emitted by infrared treated atoms

e.       NIL

5. 2- log T is a definition of (T= transmission):

    a. absorbance                                              d. electron density                  e. NIL

     b. ionic strength                                         e. electrophoretic density

6.  In Osmometry, concentration of substance can be measured by:

   a. colorimetry                                              d. freezing point depression

    b. appropriate filters                                    e. NIL

    c. ionized atoms

7.  Quenching is a problem in analysis of:

   a. ultraviolet  spectrophotometry                 d. flame photometry

   b. fluorescence                                             e. NIL

   c. atomic absorption

8.  For AAS measurements the light source is usually the:

a. hollow cathode lamp                                    d. Tungsten light bulb

b. Deuterium lamp                                            e. NIL

c. halogen lamp

9.  Electrophoresis is used to measure :

a.       ultraviolet substances                                      e. NIL

b.      infrared absorbing materials

c.       charged particles

d.      colored solutions

10.  The reagent blank corrects for absorbance caused by:

a.       the color of reagents                                       e. NIL

b.      sample turbidity

c.       bilirubin and hemolysis

d.      all of the above

11.  The internal standards in EFP, are:

1. Didymium and lithium
2. Cesium and lithium
3. Potassium and didymium
4. Cesium and didymium
5. NIL

12.  The parts of the burner assembly are the following:

1. Aspirator, atomizer, flame
2. Aspirator, ionizer, flame
3. Atomizer, emitter, flame
4. Atomizer, ionizer, flame
5. NIL

13.  The most accurate vessel in volumetric measurements is the:

a.       Erlenmeyer flask

b.      Graduated cylinder

c.       Serological pipette

d.      Volumetric flask

e.       NIL

14.  Volumetric measurements are generally used in:

1. Liquid preparations only
2. Solid and liquid preparations
3. Solid preparations only
4. AIL
5. NIL

15.  The unit of volume in volumetric measurements is:

1. Deciliter
2. Micriolter
3. Milliliter
4. Liter
5. NIL

16.  In electrophoresis, the basis of separation are the following, EXCEPT:

1. Charges of ions
2. Electrophoretic media
3. Net charge of particles
4. Concentration of ions
5. NIL

17.  The movement of ions in electrophoresis is from:

1. Negative to positive
2. Positive to negative
3. Negative to negative
4. Positive to positive
5. NIL

18.  The principle of chromatography is:

1. The movement of charged particles in an electric field
2. The involvement the solute and solvent
3. The measurement of emitted light
4. The excitation of ions in solution
5. NIL

19.  Chromatography is affected by the following factors, EXCEPT:

1. Density of particles in solution
2. Size of particle
3. Affinity of particles to chromatographic media
4. pH
5. NIL

20.  The following precautions are observed in Gravimetry, EXCEPT:

1. The balance should be adjusted to zero
2. The substance to be measured should be hydrated
3. The substance should be uncontaminated
4. The vessels used should be clean and dry
5. NIL

21.  The specific substance that causes lipemia is

1. Lipoprotein lipase
2. Chylomicrons
3. Triglycerides
4. Cholesterol
5. NIL

22.  The most predominant carbohydrate is:

1. Fatty acid
2. Glucose
3. Amino acid
4. Triglyceride
5. NIL

23.  Chromatography can be utilized in the separation of the following, EXCEPT:

1. Proteins
2. Sugars
3. Drugs
4. Glucose
5. NIL

24.  The following are effects of long standing of serum samples not separated from the cells at room temperature in glucose determination:

1.      There will be falsely decreased results.

2.      The rate of loss of glucose due to long standing at RT is 7 mg/dL/hr. if the serum is not separated from the cells.

3.      The rate of loss of glucose may be higher due to possible bacterial contamination.

4.      There will be falsely elevated values.

5.      There will be no effect on glucose values.

a.       1          b. 1 and 2        c. 1, 2 and 3    d. 1, 3 and 5    e. NIL

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