1. State Beer's law 2. What is the relationship between the intensity of color of a solution to its concentration? 3. What is the formula in solving the Cu? 4. What are the other methods in computing for your unknow concentration? 5. Give the types of wavelength and the ranges by which they occur. 6. List the specific colors for each wavelength. 7. List and describe briefly the basic components of the spectrophotometer.
1. DISCUSS THE PORTER SILBER METHOD. ( 5 PTS.) 2 DISCUSS THE DIALYSIS METHOD OF TOTAL T4 PROCEDURE. ( 5 PTS.) 3. HOW IS THE CHAIN OF CONFIDENTIALITY MAINTAINED IN DRUG ABUSE SCREENING? ( 5 PTS.)
1. Discuss the steps in a Quality Control Chart Preparation. 2. Discuss the 4 major processes in Quality Assurance Program. 3. Explain the Westgard Rules and their indications.
1. WRITE DOWN FORMULA. 2. SHOW COMPUTATIONS. 3. ENCLOSE ANSWERS IN BOXES.
ATOMIC WEIGHTS:
Na = 23 H = 1 S = 32 Cl= 35.5 O = 16
1. If you weigh 5 g NaCl solution and dissolve it in 1000 ml of diluent. ( 5 pts. each)
A. What is the Molarity? B. What is the Normality? C. What is the percent solution?
2. What is the Molarity of a 0.5 N H2SO4 solution? (5 pts.) 3. What is the dilution if you add 0.75 ml of serum to 3.0 ml of diluent? ( 5 pts.) 4. How would you prepare 1:20 serum dilution? (5 pts.) 5. How would you prepare a 0.5 % NaCl solution? (5 pts.) 6. You are asked to prepare 200 ml of the following standard concentrations from a 20 mg/dL standard stock solution: Tabulate volume of solute and solvent and also dilution. ( 5 pts. each)
A. 2 mg/dL C. 8 mg/dL B. 4 mg/dL D. 10 mg/dL E. 15 mg/dL
1. The supernatant fluid when clotted blood has been centrifuged. - SERUM 2. The supernatant fluid when anti-coagulated blood has been centrifuged.- PLASMA 3. Reference method for electrolyte determination.- ION SELECTIVE ELECTRODE 4. A process wherein a needle is introduced into a vein to obtain blood.- VENIPUNCTURE 5. Main function is water electrolyte balance.- SODIUM 6. How many minutes do you centrifuge the blood for serum preparation? - 5 TO 10 MINUTES 7. It is the precision and accuracy of a method.- RELIABILITY 8. Normal anion-gap value. - 8 to 12 mEq/L 9. The normal pH of serum.- 7.35 to 7.45 10. The most common sample for clinical chemistry - SERUM
1. The supernatant fluid when clotted blood has been centrifuged. 2. The supernatant fluid when anti-coagulated blood has been centrifuged. 3. Reference method for electrolyte determination. 4. A process wherein a needle is introduced into a vein to obtain blood. 5. Main function is water electrolyte balance. 6. How many minutes do you centrifuge the blood for serum preparation? 7. It is the precision and accuracy of a method. 8. Normal anion-gap value. 9. The normal pH of serum. 10. The most common sample for clinical chemistry.
II. MATCHING TYPE. Match the following physical and psychological effect to the substances of abuse. Choices: A - AMPHETAMINE, B- COCAINE, C- MARIJUANA
A. alertness B. impairment of motor skills C. reduce of feeling of fatigue D. dizziness E. loss of appetite F. dryness of mouth G. time perception altered H. reaction time impaired I. hallucinations J. euphoria K. blurred vision L. paranoia M. hyperactivity N. oral communication skills diminished O. vasoconstriction and elevated heart rate
1. WRITE DOWN FORMULA 1ST. 2. HOW COMPUTATIONS. 3. ENCLOSE ANSWERS IN BOXES.
1. What is the Normality of a 10 % NaCl solution? ( 5 pts.) 2. What is the Molarity of a 2 N H2SO4 solution? ( 5 pts.) 3. What is the dilution if you add o.50 ml of serum to 6.5 ml of diluent? 4. How would you prepare 1:10 serum dilution? ( 5 pts.) 5. How would you prepare a 0.85 % NaCl solution? (5 pts.) 6. Construct a Levey-Jennings chart based on the following data: (use a graphing paper) ( 5 pts.)
Control values/ standard values/ unknown values
Day 1 3.2 mmol/L 5.4 mmol/L 6.7 mmol/L Day 2 4.5 mmol/L 2.6 mmol/L 4.2 mmol/L Day 3 4.0 mmol/L 5.0 mmol/L 3.8 mmol/L Day 4 3.0 mmol/L 3.5 mmol/L 5.0 mmol/L Day 5 4.2 mmol/L 2.5 mmol/L 6.7 mmol/L Day 6 3.5 mmol/L 5.5 mmol/L 5.5 mmol/L Day 7 4.5 mmol/L 6.0 mmol/L 5.2 mmol/L Day 8 4.1 mmol/L 5.5 mmol/L 6.7 mmol/L Day 9 4.3 mmol/L 3.6 mmol/L 5.4 mmol/L Day 10 3.0 mmol/L 5.2 mmol/L 4.6 mmol/L Day 11 4.1mmol/L 4.4 mmol/L 2.7 mmol/L Day 12 6.5 mmol/L 2.6 mmol/L 5.2 mmol/L Day 13 4.0 mmol/L 5.0 mmol/L 4.8 mmol/L Day 14 2.8 mmol/L 5.5 mmol/L 5.0 mmol/L Day 15 4.2 mmol/L 2.5 mmol/L 7.7 mmol/L Day 16 5.5 mmol/L 5.5 mmol/L 2.5 mmol/L Day 17 7.5 mmol/L 7.0 mmol/L 3.2 mmol/L Day 18 3.1 mmol/L 5.5 mmol/L 2.7 mmol/L Day 19 4.3 mmol/L 4.6 mmol/L 5.4 mmol/L Day 20 5.2 mmol/L 5.2 mmol/L 3.6 mmol/L
Based on the constructed CHART: ( 5 pts.)
1. Determine if the control values are “in control” or “out of control” 2. Are there any Westgard Rules violated? If there are, encircle with red ink the violations. 3. If there are violations, Identify whether “trend” or “shift”. 4.What appropriate measures could be adapted to correct the error?
Discuss briefly but concisely the following topics.
1. The negative feedback mechanism of glucose 2. The chain of confidentiality in drug testing. 3. The principle of the OGTT. 4. The side effects of marijuana.
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